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Article Title: Conformational strictness required for maximum activity and stability of bovine pancreatic ribonuclease A as revealed by crystallographic study of three Phe120 mutants at 1.4 ? resolution

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Figure Lengend Snippet: Statistics of data collection and structure refinement

Article Snippet: The statistics of the data collection were judged to be fit for use as listed in Table 1 . table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Enzyme Wild type F120A F120G F120W Data collection Space group P3 2 21 Unit cell dimension (Å) a = b = 64.05, c = 63.36 a = b = 63.66, c = 63.49 a = b = 63.73, c = 63.49 a = b = 63.90, c = 63.33 (deg) γ = 120 γ = 120 γ = 120 γ = 120 X-ray source SPring-8 BL40B2 SPring-8 BL44B2 SPring-8 BL40B2 SPring-8 BL44B2 Wavelength (Å) 0.968641 0.7 0.7 0.7 Detector Rigaku RAXIS IV++ IP ADSC Quantum 4R CCD Rigaku RAXIS IV ++ IP ADSC Quantium 4D CCD Mount Cryo protectant (300 mg/ml trehalose) Maximum resolution (Å) 1.35 1.00 1.20 1.00 Data collection resolution (Å) 55.5–1.35 16.8–1.00 41.7–1.20 16.8–1.00 Number of total reflections 241,908 1,446,353 404,905 886,320 Number of unique reflections 33,471 80,089 41,727 80,099 Completeness (%) 98.8 99.7 88.8 99.6 Average I/σ 14.3 36.9 12.6 34.9 R merge 0.05 0.07 0.04 0.06 Structure refinement Refinement resolution (Å) 6.00–1.40 5.00–1.40 6.00–1.40 6.00–1.40 R-factor 0.204 0.201 0.210 0.218 R-free 0.242 0.254 0.265 0.272 RMS (bond distances) (Å) 1.2 1.2 1.1 1.2 RMS (bond angle) (deg) 0.006 0.005 0.005 0.005 Open in a separate window Statistics of data collection and structure refinement The structures were refined by rigid-body, positional, slow-cool, and b-refinement in X-PLOR.

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